Sulfite reductase employs siroheme rather than heme due to higher efficiency in transferring electrons incoming from the [4Fe4S] cubane cofactor

  • Date: Jan 15, 2026
  • Time: 11:00 AM - 12:00 PM (Local Time Germany)
  • Speaker: Adrian M.V. Brânzanic
  • Interdisciplinary Research Institute on Bio-Nano Sciences, Babeș-Bolyai University, Cluj-Napoca, Romania. Raluca Ripan Institute for Research in Chemistry, Babeș-Bolyai University
  • Location: MAx-Planck-Institut für Festkörperforschung
  • Room: 4D2
  • Host: Dep. of Electronic Structure Theory
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Sulfite reductase (SiR) contains in the active site a unique assembly of siroheme and a [4Fe4S] cluster, linked by a cysteine residue. Siroheme is a doubly reduced variant of heme that is not used for a catalytic function in any other enzyme. We have used1 non-equilibrium Green’s function methods coupled with density functional theory computations to explain why SiR employs siroheme rather than heme. The results show that direct, through vacuum, charge-transfer routes are inhibited when heme is replaced by siroheme. This ensures more efficient channelling of the electrons to the catalytic iron during the six-electron reduction of sulfite to sulfide, limiting potential side reactions that could occur if the incoming electrons were delocalized onto the macrocyclic ring.

References:
1A.M.V. Branzanic, U. Ryde, R. Silaghi-Dumitrescu, Chemical Communications, 2019, 55, 14047-14049.

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