Biological nanopores for single-molecule analysis

The nanopore technique is an electrophoretic approach that can identify a single molecules as they pass through a nanometer-scale pore. By measuring the ionic current changes induced by the target molecule, various chemical and physical properties - such as size, mass, composition, structure, sequence and conformation - can be obtained. This technique has become a powerful tool for single-molecule analysis in many fields, including metal ion detection, single-molecule chemistry, polymer size discrimination, nucleic acid sequencing, and protein/peptide/glycan analysis. In this presentation, I will demonstrate how we utilize and engineer biological nanopores for molecular sensing, sequencing, and addressing fundamental biophysical questions.