Nano-Bio Interfaces

Nanoscale tools serve as ideal interfaces to interrogate biological systems since the sizes of many nanostructures are comparable to that of typical biomolecules. The activities in this research direction are aimed on one hand at tailoring nanostructures to attain controlled biological interfaces, while on the other hand at characterizing biological systems with a high resolution on the nanoscale. After soft-landing under clean vacuum conditions on substrate using the unique electrospray deposition method developed in the department, individual biomolecules can be imaged by scanning probe microscopy, local magnetic resonance techniques and low energy electron holography with ultimate resolution.

Theoretical landscape methods are employed to analyze, interpret and predict the stability, the adsorption conformation and the possible self-assembly structures of biomolecules on supporting surfaces.

Responsible Scientists:

Dr. Kelvin Anggara

Dr. Luigi Malavolti

Prof. Stephan Rauschenbach – also at University of Oxford

Prof. Uta Schlickum – also at TU Braunschweig

Prof. J. Christian Schön

Team Members

Moritz Edte, Domenico Paone, Dinesh Pinto, Hannah Ochner, Dr. Johannes Seibel, Sven Szilagyi

Key Publications

Hannah Ochner,  Sven Szilagyi, Sabine Abb, Joseph Gault, Carol V. Robinson, Luigi Malavolti, Stephan Rauschenbach, and Klaus Kern
Low-energy electron holography imaging of conformational variability of single-antibody molecules from electrospray ion beam deposition
PNAS 118, e2112651118 (2021)

Kelvin Anggara, Yuntao Zhu, Giulio Fittolani, Yang Yu, Theodore Tyrikos-Ergas, Martina Delbianco, Stephan Rauschenbach, Sabine Abb, Peter H. Seeberger, and Klaus Kern
Identifying the origin of local flexibility in a carbohydrage polymer
PNAS 118, e2102168118 (2021)

Lukas Krumbein, Kelvin Anggara, Martina Stella, Tomasz Michnowicz, Hannah Ochner, Sabine Abb, Gordon Rinke, André Portz, Michael Dürr, Uta Schlickum, Andrew Baldwin, Andrea Floris, Klaus Kern, and Stephan Rauschenbach
Fast Molecular Compression by a Hyperthermal Collision Gives Bond-Selective Mechanochemistry
Phys. Rev. Lett., accepted (2021) arXiv:2007.04497

Kelvin Anggara, Yuntao Zhu, Martina Delbianco, Stephan Rauschenbach, Sabine Abb, Peter H. Seeberger, and Klaus Kern
Exploring the Molecular Conformation Space by Soft Molecule-Surface Collision
J. Am. Chem. Soc. 142, 21420 (2020)

X. Wu, M. Delbianco, K. Anggara, T. Michnowicz, A. Pardo-Vargas, P. Bharate, S. Sen, M. Pristl,
S. Rauschenbach, U. Schlickum, S. Abb, P. H. Seeberger, K. Kern
Imaging single glycans
Nature, 582, 375 (2020)

Sabine Abb, Nathalie Tarrat, Juan Cortes, Bohdan Andriyevsky, Ludger Harnau, J. Christian Schön,
Stephan Rauschenbach, Klaus Kern
Carbohydrate Self-Assembly at Surfaces: STM Imaging of Sucrose Conformation and Ordering on Cu(100)
Angewandte Chemie 131, 8424-9428 (2019)

A. Portz, M. Baur, G. Rinke, S. Abb, S. Rauschenbach, K. Kern, and M. Dürr
Chemical analysis of complex surface-adsorbed molecules and their reactions by means of cluster-induced desorption/ionization mass spectrometry
Analytical Chemistry 90, 3328 (2018)

Jean-Nicolas Longchamp, Stephan Rauschenbach, Sabine Abb, Conrad Escher, Taliana Latychevskaia, Klaus Kern, and Hans-Werner Fink
Imaging proteins at the single-molecule level
PNAS 114, 1474 (2017)

Stephan Rauschenbach, Gordon Rinke, Rico Gutzler, Sabine Abb, Alyazan Albarghash, Duy Le, Talat S Rahman, Michael Dürr, Ludger Harnau, and Klaus Kern
Two-Dimensional Folding of Polypeptides into Molecular Nanostructures
ACS Nano 11, 2420 (2017)

Sabine Abb, Ludger Harnau, Rico Gutzler, Stephan Rauschenbach, and Klaus Kern
Two-Dimensional Honeycomb Network through Sequence-Controlled Self-Assembly of Oligopeptides
Nature Commun. 7, 10335 (2016)

Laura Zuccaro, Cinzia Tesauro, Tetiana Kurkina, Paola Fiorani, Hak Ki Yu, Birgitta R. Knudsen, Klaus Kern, Alessandro Desideri, and Kannan Balasubramanian
Real-time label-free direct electronic monitoring of topoisomerase enzyme binding kinetics on graphene
ACS Nano 9, 11166 (2015)

K. Balasubramanian, K. Kern
25th Anniversary Article: Label-free electrical biodetection using carbon nanostructures
Adv. Mater. 26 (2014) 1154.

G. Rinke, S. Rauschenbach, L. Harnau, A. Albarghash, M. Pauly, and K. Kern
Active Conformation Control of Unfolded Proteins by Hyperthermal Collision with a Metal Surface
Nano Lett. 14, 5609 (2014)

S. Rauschenbach, G. Rinke, N. Malinowski, R. T. Weitz, R. Dinnebier, N. Thontasen, Z. Deng, T. Lutz, P. M. de Almeida Rollo, G. Costantini, L. Harnau and K. Kern
Crystalline Inverted Membranes Grown on Surfaces by Electrospray Ion Beam Deposition in Vacuum
Adv. Mater. 24 (2012) 2761-2767

Z. Deng, N. Thontasen, N. Malinowski, G. Rinke, L. Harnau, S. Rauschenbach and K. Kern
A Close Look at Proteins: Submolecular Resolution of Two- and Three-Dimensionally Folded Cytochrome c at Surfaces
Nano Lett. 12 (2012) 2452-2458

K. Balasubramanian, T. Kurkina, A. Ahmad, M. Burghard and K. Kern
Tuning the functional interface of carbon nanotubes by electrochemistry: Towards nanoscale chemical and biosensors
J. Mater. Res. (2012)
T. Kurkina, A. Vlandas, A. Ahmad, K. Kern and K. Balasubramanian
Label-free detection of few copies of DNA with carbon nanotube impedance biosensors
Angew. Chem. Intl. Ed. 50, 3710 (2011)
A. Vlandas, T. Kurkina, A. Ahmad, K. Kern and K. Balasubramanian
Enzyme-free sugar sensing in microfluidic channels with an affinity based single-wall carbon nanotube sensor
Anal. Chem. 82, 6090 (2010)

S. Rauschenbach, F. L. Stadler, E. Lunedei, N. Malinowski, S. Koltsov, G. Costantini & K. Kern
Electrospray Ion Beam Deposition of Clusters and Biomolecules
Small 2, 540-547 (2006)

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